Fluorometric Titration for Serum Chloride

Fluorometric method for measuring serum lipase activity.

We describe a method for determining lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3) activity in serum. Emulsified olive oil in tris(hydroxymethyl)aminomethane buffer is used as substrate. The course of the enzymatic reaction is followed by measuring the decrease in fluorescence with time of a fluorescent indicator, 4-methylumbelliferone, added to the substrate, caused by the change in the ...

Electrometric Titration of Chloride in Small Volumes

A Convenient Method for Potentiometric Titration of Chloride Ions

A convenient reference cell and method of potentiometric determination of chloride ions with silver nitrate is described. The method is accurate to about +/- 0.02 ml. 10(-4)M silver nitrate.

Kinetic fluorometric determination of aluminum in serum.

We describe a simple fluorometric method for determining aluminum in serum samples by monitoring the rate of reaction of 2-hydroxy-1-naphthaldehyde-p-methoxybenzoylhydrazone with aluminum ions. The emission of the resulting fluorescent metal-chelate formed is measured at 475 nm. Aluminum was measured in the supernate of serum after proteins were removed by precipitation with concentrated nitric...

Fluorometric liquid-chromatographic determination of serum cortisol.

We describe a sensitive and specific liquid-chromatographic assay for serum cortisol, exploiting acid-induced fluorescence. Each analysis requires only 50 microL of serum, and chromatography is complete in 7 min. Analytical recovery of cortisol added to serum ranged from 90 to 105% and between-run precision (CV) from 6 to 8%. The lower limit of detection for cortisol is 10 micrograms/L, and lin...